Selection of internal standards for accurate quantification of complex lipid species in biological extracts by electrospray ionization mass spectrometry - what, how and why?
행사&학회소개
II. PRINCIPLE OF QUANTIFICATION OF COMPLEX LIPID SPECIES WITH MS
III. PLATFORMS FOR QUANTIFICATION OF COMPLEX LIPID SPECIES
A. Quantification With LC-Coupled ESI-MS
B. Quantification With Direct Infusion ESI-MS (Shotgun Lipidomics)
IV. FACTORS THAT AFFECT ACCURATE QUANTIFICATION OF COMPLEX LIPID SPECIES IN LIPIDOMICS
A. Lipid Aggregation
B. Effects of 13Isotopes on Quantification
C. Ionization Efficiency
D. Ion Suppression
E. Dynamic Range
F. Tandem MS Factor
G. In-Source Fragmentation
H. Matrix Effects
V. SELECTION OF INTERNAL STANDARDS
A. Why Internal Standards Are Required for Accurate Quantification
B. What Kind of Lipid Species Can Be Used as Internal Standards
C. How Many Internal Standards Are Necessary and Why
D. How Much Individual Internal Standard Should Be Used
VI. SUMMARY