동향

Regulation of Apoptosis by Caspases

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Jynying Yuan


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Caspases are a family of cysteine proteases that play important roles in regulating apoptosis, a genetically encoded cellular suicide mechanism. To determine the mechanism by which caspases regulate apoptosis. We carried out a screen to identify substrates of caspases using small pool expression cloning method. We have screened 1700 pools which contains approximately 70,000 cDNA clones. From such screening, we identified more than 60 caspase substrates, 10 of which have been verified to be cleaved in vivo during apoptosis. Of these substrates, 24 have not been previously identified or have no assigned function.
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