KOSEN 한인과학기술자네트워크

다음 논문들은 PubMed를 검색하여 찾은 것입니다. 1: Methods Mol Biol 2000;147:129-39 Dye-ligand affinity chromatography for protein separation and purification. Labrou NE Department of Biochemistry and Molecular Biology, University of Leeds, UK. No abstract available. 2: J Mol Recognit 1996 Sep-Dec;9(5-6):564-9 Protein A mimetic peptide ligand for affinity purification of antibodies. Fassina G, Verdoliva A, Odierna MR, Ruvo M, Cassini G TECNOGEN S.C.p.A., Piana di Monte Verna (CE), Italy. A peptide mimicking protein A for its ability to recognize the Fc immunoglobulin portion has been identified through screening of a synthetic multimeric peptide library. Screening of the multimeric library, composed of randomized synthetic tripeptide tetramers, has been carried out using a very simple assay, measuring the library ability to interfere with the interaction between protein A and biotinylated immunoglobulins, monitored on solid phase using an enzyme-linked immunosorbent assay format. The tetrameric tripeptide identified after three screening cycles was produced in larger amounts and then immobilized in high yield on preactivated solid support for the preparation of affinity columns, which proved useful for a very convenient one-step purification of antibodies directly from crude sera. Antibody purity after affinity purification was close to 95 per cent, as determined by densitometric scanning of sodium dodecyl sulphate-polyacrylamide gel electrophoresis gels of purified fractions, and up to 2 mg of antibody could be purified from 1 ml of peptide-derivatized affinity support. The ligand was stable to treatment with a vast array of sanitation agents, such as ethanol and 0.1 M sodium hydroxide, and to repeated use, thus making the ligand applicability extremely attractive for the purification of monoclonal antibodies for therapeutic use. Column binding selectivity was similar to that of protein A-affinity columns, since immunoglobulin G from several sources (rabbit, goat, sheep, mouse) was conveniently purified, with no detection of leaked ligand fragments in the purified preparations. PMID: 9174941, UI: 97317958 3: J Biochem (Tokyo) 1995 Feb;117(2):443-6 Use of an SDS-gel-separated protein band as a ligand for affinity chromatography: procedure and application to the purification of domain-specific antibodies against alpha-actinin. Yoshihara Y, Kuroda M Department of Biology, Faculty of Sciences, Shimane University. This paper describes a simple and efficient method for preparing affinity columns. We used protein separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis as a ligand. Protein bands detected in a polyacrylamide gel were electrophoretically transferred to CNBr-activated Sepharose using a buffer containing Nonidet P-40. The amount of ligand protein coupled to activated Sepharose by our method was almost comparable to that obtained by conventional coupling procedure with a native ligand protein. Using affinity columns prepared by this method, we have successfully purified anti-alpha-actinin antibody and antibodies highly specific to the rod domain of alpha-actinin from the antiserum. This new method should be useful for separating a specific antibody from an antiserum that has been raised against multiple antigens. In addition, the use of the SDS-gel-fractionated band facilitates the coupling of proteins that have low solubility under the coupling conditions. PMID: 7608136, UI: 95332273 4: Anal Biochem 1999 Oct 1;274(1):118-24 Related Influence of the antibody purification method on immunoassay performance: hapten-antibody binding in accordance with the structure of the affinity column ligand. Choi J, Kim C, Choi MJ Bioanalysis & Biotransformation Research Center, Korea Institute of Science and Technology, Seoul, 130-650, Korea. jechoi@kistmail.kist.re.kr The effects of ligands for immunoaffinity chromatography on the immunoassay were investigated with three goat anti-methamphetamine (anti-MA) antibodies (Abs). An N-4-aminobutyl derivative of methamphetamine (4-ABMA) was conjugated with proteins and used as immunogens. All the antisera produced were purified by affinity chromatography with various ligands of 4-ABMA-proteins and of haptens as well as protein G: 4-ABMA-bovine serum albumin (4-ABMA-BSA), 4-ABMA-keyhole limpet hemocyanine (4-ABMA-KLH), 4-ABMA-ovalbumin (4-ABMA-OVA), MA, 4-ABMA, and amphetamine were used as ligands. Enzyme-linked immunosorbent assay (ELISA) was conducted to examine characteristics of the purified Abs with the 4-ABMA-OVA competitor coated. The results obtained revealed that characters of the purified Abs were closely related with chemical structures of ligands used. The Abs from the MA and the amphetamine columns showed better sensitivities than those from the others in each antiserum. Particularly, the Ab from the amphetamine column gave the best results in terms of sensitivity and specificity. The recognition or the affinity of the Ab selected was considered to be affected by the structure of the ligand concerned. These results suggest that the Ab purification method should be considered as an important parameter which has great influence on the performance of immunoassays with polyclonal Abs. Copyright 1999 Academic Press. PMID: 10527504, UI: 99459234 5: J Mol Recognit 1998 Winter;11(1-6):128-33 Immunoglobulin specificity of TG19318: a novel synthetic ligand for antibody affinity purification. Fassina G, Verdoliva A, Palombo G, Ruvo M, Cassani G TECNOGEN SCpA, Piana di Monte Verna (CE), Italy. fassina@tecnogen.it A synthetic ligand [TG19318], able to mimic protein A in the recognition of the immunoglobulin Fc portion, has been previously identified in our laboratory through the synthesis and screening of multimeric combinatorial peptide libraries. In this study we have fully characterized its applicability in affinity chromatography for the downstream processing of antibodies, examining the specificity and selectivity for polyclonal and monoclonal immunoglobulins derived from different sources. Ligand specificity was broader than protein A, since IgG deriving from human, cow, horse, pig, mouse, rat, rabbit, goat and sheep sera, IgY obtained from egg yolk, and IgM, IgA and IgE were efficiently purified on TG19318 affinity columns. Adsorbed antibodies were conveniently eluted by a buffer change to 0.1 M acetic acid or 0.1 M sodium bicarbonate pH 9, with full retention of immunological properties. Monoclonal antibodies deriving from cell culture supernatants or ascitic fluids were also conveniently purified on TG19318 affinity columns, even from very diluted samples. The affinity constant for the TG19318-IgG interaction was 0.3 microM, as determined by optical biosensor measurements. Under optimized conditions, antibody purity after affinity purification was close to 95%, as determined by densitometric scanning of SDS-PAGE gels of purified fractions, and maximal column capacity reached 25 mg Ig/ml support. In vivo toxicity studies in mice indicated a ligand oral toxicity greater than 2000 mg kg-1 while intravenous toxicity was close to 150 mg kg-1. Validation of antibody affinity purification processes for therapeutic use, a very complex, laborious and costly procedure, is going to be simplified by the use of TG19318, which could reduce considerably the presence of biological contaminants in the purified preparation, a very recurrent problem when using recombinant or extractive biomolecules as affinity ligands. PMID: 10076825, UI: 99176121 6: J Chromatogr 1992 Apr 24;597(1-2):101-9 Avid AL , a synthetic ligand affinity gel mimicking immobilized bacterial antibody receptor for purification of immunoglobulin G. Ngo TT, Khatter N BioProbe International, Inc., Tustin, CA 92680. Avid AL is an affinity gel designed for the purification of immunoglobulin G (IgG). The gel was prepared by first reacting Sepharose with 3,5-dichloro-2,4,6-trifluoropyridine and 4-dimethylaminopyridine and then with 2-mercaptoethanol. The IgG purified by Avid AL is about 95% pure. The binding parameters of Avid AL for the whole IgG, Fab and Fc fragment and the stability of gel were investigated. The IgG bound to Avid AL can be eluted with an acidic buffer or with a novel neutral buffer containing electron donors. The development of such a mild neutral elution buffer is described. Application of Avid AL in a rapid gram-scale IgG purification was demonstrated. The possible mechanism of IgG binding is discussed. PMID: 1517307, UI: 92388282 7: Diabetes Res Clin Pract 1985 Dec;1(4):235-41 Purification of insulin-binding antibody by affinity chromatography using monocomponent insulin as ligand. Miyai Y, Okada S, Ota Z Insulin-binding antibody (IBA) was purified by affinity chromatography using porcine monocomponent (MC) insulin as the ligand. The purity of the antibody was compared with that of the antibody extracted using porcine crystalline (Cr) insulin. Comparing the antibody solutions obtained with MC insulin (MC-lig-sol) or Cr insulin (Cr-lig-sol), the content of IBA in Cr-lig-sol was higher than in MC-lig-sol, but the content of proinsulin-binding antibody (PBA) in MC-lig-sol was very small and statistically lower than that in Cr-lig-sol (P less than 0.01). Adding native MC insulin to a competitive radioimmunoassay suppressed the IBA titer obtained with MC insulin more than that obtained with Cr insulin. By adding native proinsulin in a similar assay system, the PBA titer obtained with Cr insulin was suppressed more than that extracted with MC insulin. Scatchard analysis of the 2 solutions showed that the affinity constants of high affinity antibodies were almost identical, but that of low affinity antibody in MC-lig-sol was larger than in Cr-lig-sol. The binding capacity of low affinity antibody in Cr-lig-sol was 15 times as much as that in MC-lig-sol. Using MC insulin, instead of Cr insulin, as the ligand in affinity chromatography increased the purity of recovered IBA. chromatography increased the purity of recovered IBA. PMID: 3915264, UI: 86246946

Europe patent office(http://ep.espacenet.com/)에서 antibody carrier라는 검색어로 찾은 특허 중 1째 페이지 목록입니다. 총 25 페이지 617 특허 정도 됩니다. 그런데 단백질 정제라는 것인 워낙 case by case이기 때문에 보다 특정 단백질에 대한 정확한 정보를 얻고 싶으시면 보다 자세히 적어 주셔야 할 것 같습니다. WO0061761 RECOMBINANT TOXIN A PROTEIN CARRIER FOR POLYSACCHARIDE CONJUGATE VACCINES US6124105 Method for detecting the presence of a mycobacterium species and a kit and antibodies for use therein BG103808 METHODS AND COMPOSITIONS FOR MODULATION RESPONSIVENESS TOCORTICOSTEROIDS US6114179 Method and test kit for detecting antigens and/or antibodies CN1261670 No title available. CN1259524 No title available. CN1260490 No title available. JP11092400 MONOCLONAL ANTIBODY AND VACCINE AGAINST HUMAN CANCER-RELATED ANTIGEN BY IMMUNITY USING ANIMAL AND HUMAN MUTIN AND USING SYNTHETIC CARBOHYDRA WO0044403 HER-2 BINDING ANTAGONISTS WO0037941 A METHOD OF DETECTING AND/OR QUANTIFYING A SPECIFIC IgE ANTIBODY IN A LIQUID SAMPLE WO0029620 METHODS OF FACILITATING VASCULAR GROWTH WO0027435 ANTIBODY-SERUM PROTEIN HYBRIDS WO0026667 VARIABLE HEAVY CHAIN AND VARIABLE LIGHT CHAIN REGIONS OF ANTIBODIES TO HUMAN PLATELET GLYCOPROTEIN IB ALPHA WO0021559 BLOCKING FACTOR B TO TREAT COMPLEMENT-MEDIATED IMMUNE DISEASE WO0020606 METHOD FOR THE PRODUCTION OF (POLY)PEPTIDES BY USING TRUNCATED VARIANTS OF THE SV40 LARGE T ANTIGEN WITH AN INTACT N TERMINUS WO0014538 LINKER-ASSISTED IMMUNOASSAY FOR GLYPHOSATE WO0010388 SIALIDASES AS MUCOSAL ADJUVANTS WO0009525 LOW ADENOSINE ANTI-SENSE OLIGONUCLEOTIDE AGENT, COMPOSITION, KIT AND TREATMENTS WO0006203 DNA CYTOKINE VACCINES AND USE OF SAME FOR PROTECTIVE IMMUNITY AGAINST MULTIPLE SCLEROSIS WO0003730 UREASE BASED VACCINE AGAINST HELICOBACTER INFECTION

> 안녕하세요. 경희대학교에서 화학공학, 유전공학을 전공하고 있는 노정권이라고 합니다. 제가 특히 알고 싶은 것을 좀 더 특화시킨다면, 특정 단백질을 막분리할 때에 쓰이는 affinity carrier를 만드는 방법입니다. 그 방법에 다른 것보다 antibody가 들어간다면 더욱 도움이 되겠네요. > 주신 자료를 가지고 정말 열심히 공부해서 저도 남들 공부에 도움이 되는 사람이 되도록 하겠습니다. 감사합니다. ----------------------------------------------------------------------- These papers were published on your requested subject. ---------------------------------------------------------------- Gas transport properties of HTPB based polyurethane/cosalen membrane Chen, Shih-Hsiung (Chia-Nan Coll of Pharmacy and Science) Yu, Kuang-Chang | Houng, Shih-Liang | Lai, Juin-Yih Source: Journal of Membrane Science 173 1 2000 Elsevier Science Publishers B.V. p 99-106 0376-7388 Abstract: Polyurethane (PU) membranes own high gas permeability but low selectivity. In the past, some attempts to increase the gas selectivity by modifying the polymer structure but the effort were unsuccessful. There were other attempts to improve the gas separation by the addition of high affinity salt into a membrane. It was reported that increasing the amount of oxygen carrier salt (cosalen) into a polycarbonate membrane did increase the selectivity of oxygen to nitrogen especially at low temperature. An increase in salt addition reduced the gas diffusivity but increased the diffusivity ratio of oxygen to nitrogen. In another study, it was also found that the addition of high oxygen-affinity salt into a polycarbonate membrane did not greatly increase the solubility ratio of oxygen to nitrogen but it significantly increased the diffusivity ratio of oxygen to nitrogen. In this study, the effects of oxygen carrier salt namely, cosalen, in PU membrane on gas separation performance was examined. Gas diffusion and sorption properties were examined by sorption measurements and dual mode analyses. The dual mode analysis showed that the gas separation in PU membrane was dominated by gas diffusion rather than gas sorption. The selectivity of O2/N2 was 8.6 and the oxygen permeability was 1.1 barrer for the PU membrane with 5 wt.% cosalen at 5 °C. The key issue of improving the gas separation performance of a polyurethane membrane was to increase the diffusivity ratio but not the solubility ratio. In English 14 Refs. 2. Poisoning of liquid membrane carriers in extraction of metal ions Wang, Yuchun (Jiangxi Normal Univ) Wang, Dexian Source: Separation Science and Technology v 27 n 3 Mar 1992 p 341-347 0149-6395 Abstract: In the extraction of desired metal (scandium, mixed rare earths) ions using chelating extractants (TTA, HDEHP) as liquid membrane carriers, the carriers will become poisoned owing to the presence of even minute quantity of certain high ionic potential ions in the feed solution. The reason for the poisoning of carrier is that those ions have so much greater affinity than the desired ions for the membrane carrier that the ion-carrier coordination compound cannot be stripped at the interior interface of the membrane and gradually no more free carrier transports any metal ions across the membrane. The calculated results are in agreement with the experiments, and methods to avoid the poisoning are given in the paper. In English 7 Refs EI92050480270 3. Two-dimensional protein crystals (S-layers): fundamentals and application potential Sleytr, Uwe B. (Universitat fur Bodenkultur) Sara, M. | Pum, D. | Kupcu, S. | Messner, P. Source: Materials Research Society Symposium Proceedings v 330 Nov 29-Dec 3 1993 1994 Materials Research Society p 193-199 0272-9172 Abstract: Crystalline cell surface layers (S-layers) represent the outermost cell envelope component in many bacteria. The oblique, square or hexagonal lattices are formed of assemblies of a single protein or glycoprotein species. Isolated S-layer subunits are endowed with the ability to assemble into monomolecular arrays in suspension, on solid surfaces (e.g. metals, polymers, glass, carbon, silicon), at the air/water interface, or on lipid films generated by the Langmuir Blodgett technique. S-layer lattices are isoporous structures with functional groups located on the surface and in the pores in an identical position and orientation. These characteristic features have led to applications of S-layers as (i) ultrafiltration membranes with pores of identical size and morphology and a broad chemical modification potential, (ii) matrices for the controlled and reproducible immobilization of functional macromolecules, as required for affinity and enzyme membranes, affinity microcarriers and biosensors, (iii) carriers for Langmuir-Blodgett films and reconstituted biological membranes, (iv) immobilization matrices and adjuvants for weakly immunogenic antigens and haptens and (v) patterning elements in molecular nanotechnology. In English 36 Refs. EI94122453024 4. Two-component emulsion liquid membranes with macromolecular carriers of divalent ions Wodzki, Romuald (Nicolaus Copernicus Univ) Wyszynska, Alicja | Narebska, Anna Source: Separation Science and Technology v 25 n 11-12 Sep-Oct 1990 p 1175-1187 0149-6395 Abstract: Experimental investigations are presented on the application of poly[poly(ethylene glycol) phosphate] (PEGP) as a macromolecular, multisite, carrier of ions in an emulsion liquid membrane system. This polymer acts as a mobile carrier and surfactant-stabilizing W/O/W emulsion. The transport properties of PEGP have been checked for systems composed of (a) a feed phase containing Ni(II), Co(II) or Cu(II) ions; (b) an organic phase, i.e., PEGP dissolved in 1,2-dichloroethane; and (c) 1 M sulfuric acid as a receiving solution. Fast and effective membrane extraction of Ni(II) has been found for membranes with PEGP of molecular weight 22,000. It has been proved that the affinity of PEGP toward the studied ions is reversed in comparison with that for poly(ethylene glycol). The affinity order of PEGP toward the tested ions is Ni(II) gt; Co(II) gt; Cu(II). In English 16 Refs EI91010091535 5. Recent developments in the use of group-specific ligands for affinity bioseparations Ramirez-Vick, Jaime E. (Arizona State Univ) Garcia, Antonio A. Source: Separation and Purification Methods v 25 n 2 1996-1997 Marcel Dekker Inc p 85-129 0360-2540 Abstract: Group specific ligands, which can be of two types, the biological ligands and the synthetic ligands, have been effectively introduced into the bioseparation process due to their versatility and cost effectiveness. These ligands are used in the separation process by immobilizing them onto a carrier or support. However, more work for the applications of these ligands is needed to overcome problems regarding toxic effects of the dyes or metal ion solubility. The applications of the group specific ligand in precipitation; ultrafiltration; extraction using two phase aqueous reverse micelle and perfluorocarbon systems; chromatography and membrane separation are discussed. In English 107 Refs. EI97083787250 6. Polycarbonate/N,N prime -dialicylidene ethylene diamine) cobalt(II) complex membrane for gas separation Chen, Shih Hsiung (Chung Yuan Univ) Lai, J.Y. Source: Journal of Applied Polymer Science 59 7 Feb 14 1996 John Wiley & Sons Inc p 1129-1135 0021-8995 Abstract: A polycarbonate/N,N prime -dialicylidene ethylene diamine) cobalt(II) (cosalen) complexed membrane was prepared by introducing oxygen carrier (cosalen) into polycarbonate for gas separation. With increasing amount of oxygen carrier in the membranes, the selectivity of O2/N2 increased and the permeabilities of both O2 and N2 decreased. The selectivity of O2/N2 decreased with increasing operating temperature. The pure gases sorption measurements indicated that the affinity between oxygen and the membranes was appreciable higher than that of nitrogen. According to the X-ray analysis of the complexed membranes, the decreased gas diffusivities were caused by the increase of the packing density. The selectivity of O2/N2 was 15 and oxygen permeability for the cobalt complexed membrane with 3 wt % oxygen carrier was 0.33 barrers at 5°C. Furthermore, a dual mode sorption mechanism was utilized to describe the behavior of gas sorption and permeation through the cobalt complexed membrane. In English 17 Refs. EI96023034649 7. Diffusional phenomena in membrane separation processes van den Berg, G.B. (Unilever Research) Smolders, C.A. Source: Journal of Membrane Science v 73 n 2-3 Oct 9 1992 p 103-118 0376-7388 Abstract: Nowadays membrane filtration processes are used industrially as an alternative to conventional separation methods. Membrane separation methods can be divided into classes according to their separation characteristics: (i) separation by sieving action; (ii) separation due to a difference in affinity and diffusivity; (iii) separation due to a difference in charge of molecules; (iv) carrier-facilitated transport, and (v) the process of (time-) controlled release by diffusion. In all these cases diffusion processes play an important role in the transport mechanism of the solutes. Various mechanisms have been distinguished to describe the transport in membranes: transport through bulk material (dense membranes), Knudsen diffusion in narrow pores, viscous flow in wide pores or surface diffusion along pore walls. In practice, the transport can be a result of more than only one of these mechanisms. For all of these mechanisms models have been derived. The characteristics of a membrane, e.g. its crystallinity or its charge, can also have major consequences for the rate of diffusion in the membrane, and hence for the flux obtained. Apart from the diffusion transport processes in membranes mentioned above, other important diffusion processes are related to membrane processes, viz diffusion in the boundary layer near the membrane (concentration polarization phenomena) and diffusion during membrane formation. The degree of concentration polarization is related to the magnitude of the mass transfer coefficient which, in turn, is influenced by the diffusion coefficient. The effect of concentration polarization can be rather different for the various membrane processes. The phase inversion membrane formation mechanism is determined to a large extent by the kinetic aspects during membrane formation, which are diffusion of solvent and of non-solvent and the kinetics of the phase separation itself. In English 30 Refs EI93020692696 8. Membrane filtration affinity purification (MFAP) of dehydrogenases using cibacron blue Ling, T.G.I. (Univ of Lund) Mattiasson, B. Source: Biotechnology and Bioengineering v 34 n 10 Dec 5 1989 p 1321-1325 0006-3592 Abstract: The method for purification of biomolecules by a combination of affinity interactions and membrane filtration for separation of unwanted material has been found to be of interest for large-scale work. This study examines the suitability of silica nanoparticles as carriers in the process. Alcohol dehydrogenase and lactate dehydrogenases were chosen as target molecules to be purified. The binding capacity was found to be comparative to what is obtained for high-performance liquid chromatography (HPLC) packing material. Both binding and desorption of the enzymes were found to be effective. The limiting factor of the process was the filtration flow rate. In English 15 Refs 9. Separation of cobalt and nickel by liquid surfactant membranes containing a synthesized cationic surfactant Kakoi, Takahiko (Kyushu Univ) Ura, Tsuyoshi | Kasaini, Henry | Goto, Masahiro | Nakashio, Fumiyuki Source: Separation Science and Technology v 33 n 8 Jun 1998 Marcel Dekker Inc p 1163-1180 0149-6395 Abstract: Separation of cobalt(II) and nickel(II) by using a hydroxyoxime extractant has been investigated both in liquid-liquid extraction and in a liquid surfactant membrane (LSM) system. In the liquid-liquid equilibrium extraction studies, hydroxyoximes showed significant extractability for nickel ions, although LIX 84 was found to have exceptional chelating affinity for nickel ions. In the LSM system functionalized by hydroxyoxime, the cobalt ions were efficiently separated from nickel ions as a result of slower permeation of nickel chelates across the emulsion membrane. More complete cobalt recovery was achieved in the LSMs dosed with LIX 860 than when the same carrier was applied to the liquid-liquid extraction system. Furthermore, cobalt permeation rate was enhanced threefold when a quaternary ammonium type of cationic surfactant was used as an emulsifier due to carrier interaction with surfactant at the reaction interface. The permeation mechanism of ions in LSMs was elucidated by an interfacial reaction model which took into account the adsorption of the carrier and surfactant at the reaction interface. In English 12 Refs. EI98074282006 10. Efficient transport of aromatic amino acids by sapphyrin-lasalocid conjugates Sessler, Jonathan L. (Univ of Texas at Austin) Andrievsky, Andrei Source: Chemistry - A European Journal 4 1 Jan 1998 p 159-167 0947-6539 Abstract: The synthesis and characterization of several sapphyrin-lasalocid conjugates is reported. This family of receptors is capable of acting as efficient and selective carriers for aromatic α-amino acids, as judged from both U-tube and W-tube through-model-membrane transport experiments. The first member of this family, system 6, was found to display an inherent preference for phenylalamine>tryptophan>tyrosine. Further, L-amino acids were shown to be transported with greater efficiency than the corresponding D-enantiomers by this particular carrier. The high level of amino acid carrier capability displayed by receptor 6 in dichloromethane solutions correlates well with the results of equilibrium binding studies carried out using visible-spectroscopic titrations. These latter studies revealed that system 6 does display significant affinity for zwitterionic amino acids in this organic solvent. These binding studies, as well as a number of control experiments involving, inter alia, porphyrin-lasalocid conjugate 7, showed the importance of having both the sapphyrin and lasalocid subunits contained within the same overall receptor framework. The four other second-generation sapphyrin-lasalocid conjugates reported here (11-14) were also tested as carriers for the transport of Phe, Trp, and Tyr. It was found that the esterified systems 11 and 12 functioned well as amino acid carriers, while the free-acid compounds 13 and 14 did not. These latter conjugates, containing both carboxylic acid and sapphyrin subunits, presumably undergo self-assembly in-organic solutions, a process that hampers their ability to act as effective carriers. In the case of the functioning systems 11 and 12, the configuration of the stereogenic phenylalanine appendages could be varied such that either the L- or D-antipodes of the aromatic amino acid substrates being studied were transported at a greater rate. In English EI98024073695 11. Efficient transport of aromatic amino acids by sapphyrin-lasalocid conjugates Sessler, Jonathan L. (Univ of Texas at Austin) Andrievsky, Andrei Source: Chemistry - A European Journal 4 1 Jan 1998 p 159-167 0947-6539 Abstract: The synthesis and characterization of several sapphyrin-lasalocid conjugates is reported. This family of receptors is capable of acting as efficient and selective carriers for aromatic α-amino acids, as judged from both U-tube and W-tube through-model-membrane transport experiments. The first member of this family, system 6, was found to display an inherent preference for phenylalaminegt;tryptophangt;tyrosine. Further, L-amino acids were shown to be transported with greater efficiency than the corresponding D-enantiomers by this particular carrier. The high level of amino acid carrier capability displayed by receptor 6 in dichloromethane solutions correlates well with the results of equilibrium binding studies carried out using visible-spectroscopic titrations. These latter studies revealed that system 6 does display significant affinity for zwitterionic amino acids in this organic solvent. These binding studies, as well as a number of control experiments involving, inter alia, porphyrin-lasalocid conjugate 7, showed the importance of having both the sapphyrin and lasalocid subunits contained within the same overall receptor framework. The four other second-generation sapphyrin-lasalocid conjugates reported here (11-14) were also tested as carriers for the transport of Phe, Trp, and Tyr. It was found that the esterified systems 11 and 12 functioned well as amino acid carriers, while the free-acid compounds 13 and 14 did not. These latter conjugates, containing both carboxylic acid and sapphyrin subunits, presumably undergo self-assembly in-organic solutions, a process that hampers their ability to act as effective carriers. In the case of the functioning systems 11 and 12, the configuration of the stereogenic phenylalanine appendages could be varied such that either the L- or D-antipodes of the aromatic amino acid substrates being studied were transported at a greater rate. In English EI98024073695 12. Protein separation using affinity binding 1. Polystyrene core-shell latex as ligand carrier Gebben, B. (Akzo Research Lab Arnhem) van Houwelingen, G.D.B. | Zhang, W. | van den Boomgaard, Th. | Smolders, C.A. Source: Colloids and Surfaces B: Biointerfaces 3 1-2 Sep 30 1994 Elsevier Science Publishers B.V. p 75-84 0927-7765 Abstract: Polystyrene core-shell latex particles are introduced as affinity ligand carriers for affinity separations. The particles, prepared by a seeded emulsion polymerisation process, are submicron in size and composed of a hard polystyrene core surrounded by a hydrophilic shell to which affinity ligands can be covalently coupled. The reactive dye Cibacron Blue is studied as model ligand. This dye ligand, which is covalently coupled directly to hydroxyl groups on the particle surface, is studied and the amount of coupled dye is determined quantitatively by diffuse reflection spectroscopy. The degree of coupling can be controlled by the ionic strength of the reaction medium. The adsorption of bovine serum albumin to the latices appears to be proportional to the ligand density. The functionalised core-shell latices show a high colloidal stability, fast protein adsorption/desorption kinetics and a low non-specific adsorption. The latex particles can find use in affinity separation techniques such as affinity chromatography and affinity membrane filtration. In English 22 Refs. EI95012520523 13. N-Acetyl-β-D-glucosaminyl-binding properties of the envelope glycoprotein of human immunodeficiency virus typel Gattegno, Liliane (Faculte de Medecine Paris-Nord) Sadeghi, Hoss | Saffar, Line | Bladier, Dominique | Clerget-Raslain, Brigitte | Gluckman, Jean-Claude | Bahraoui, Elmostafa Source: Carbohydrate Research v 213 Jun 25 1991 p 79-93 0008-6215 Abstract: The effect of carbohydrate structures on the adsorption of HIV-1 or of recombinant envelope glycoprotein gp 160 (rgp 160) to cells of the CEM line was investigated with an indirect immunofluorescence assay using gp 120-specific mouse monoclonal antibodies (mAbs) directed to envelope gp 120. The β-D-galactosyl, α-D-mannosyl, β-D-glucosyl, N-acetyl-β-D-glucosaminyl, sialosyl, and L-fucosyl derivatives tested had no effect on this binding. However, preincubation of HIV-1 (or rgp 160) with the neoglycoprotein, β-D-GlcNAc47-BSA, specifically inhibited the labeling, by some of the mAb used, of HIV-1 (or rgp 160) bound at the cell membrane. This inhibition occurred only with mAbs that were specific for the immunodominant 'neutralizing' third variable region (V3) of gp 120. Competition for the binding to rgp 160 between β-D-GlcNAc47-BSA and mAb was further demonstrated by use of affinity matrices substituted with one of the relevant mAb (110-4), or with β-D-GlcNAc47-BSA. Besides β-D-GlcNAc47-BSA- Sepharose, rgp 160 also bound with low affinity, but high specificity, to two other N-acetyl-β-D-glucosaminyl affinity matrices, β-D-GlcNAc-divinylsulfone-agarose and asialoagalactothyroglobulin-agarose. Conversely, β-D-[125I]GlcNAc47-BSA bound specifically to gp 160-Sepharose. These results indicated that rgp 160 behaves as a N-acetyl-β-D-glucosaminyl-binding protein for GlcNAc residues presented at high density on a carrier, the carbohydrate-binding site of which is close to, or located on the V3 region of gp 120. In English 38 Refs EI91120371764 14. Protein separation using affinity binding 1. Polystyrene core-shell latex as ligand carrier Gebben, B. (Akzo Research Lab Arnhem) van Houwelingen, G.D.B. | Zhang, W. | van den Boomgaard, Th. | Smolders, C.A. Source: Colloids and Surfaces B: Biointerfaces 3 1-2 Sep 30 1994 Elsevier Science Publishers B.V. p 75-84 0927-7765 Abstract: Polystyrene core-shell latex particles are introduced as affinity ligand carriers for affinity separations. The particles, prepared by a seeded emulsion polymerisation process, are submicron in size and composed of a hard polystyrene core surrounded by a hydrophilic shell to which affinity ligands can be covalently coupled. The reactive dye Cibacron Blue is studied as model ligand. This dye ligand, which is covalently coupled directly to hydroxyl groups on the particle surface, is studied and the amount of coupled dye is determined quantitatively by diffuse reflection spectroscopy. The degree of coupling can be controlled by the ionic strength of the reaction medium. The adsorption of bovine serum albumin to the latices appears to be proportional to the ligand density. The functionalised core-shell latices show a high colloidal stability, fast protein adsorption/desorption kinetics and a low non-specific adsorption. The latex particles can find use in affinity separation techniques such as affinity chromatography and affinity membrane filtration. In English 22 Refs. EI95012520523 15. Oxidation of cyclic alcohols from an aqueous solution by manganese porphyrins embedded in a polydimethylsiloxane membrane Neys, P.E.F. (Centrum voor Oppervlaktechemie en Katalyse) Vankelecom, I.F.J. | Parton, R.F. | Dehaen, W. | L'abbe, G. | Jacobs, P.A. Source: Journal of Molecular Catalysis, A: Chemical 126 1 Dec 1 1997 p L9-L12 1381-1169 Abstract: The use of [5,10,15,20-tetrakis(2,6-dichlorophenyl)porphyrinato] manganese(III) chloride [TDCPP(Mn)Cl] embedded in polydimethylsiloxane (PDMS) is reported for the oxidation of cyclic alcohols to ketones with t-butylhydroperoxide from an aqueous solution. Two important observations are made which both can be ascribed to the sorption exercised by the polymer. Firstly, compared to a homogeneous set-up much higher activities are reached with the PDMS-system. Secondly, the increase in activity is more pronounced as the ring size of the alcohol increases. The increase in TON going from a five ring to a seven ring is related to the sorption of the respective alcohols in the membrane. Thus, PDMS can be considered as a support with a threefold function. Besides immobilizing and dispersing the complexes, the carrier takes part in the reaction process by selectively sorbing the reagents. Furthermore, the catalyst retained its activity in a second run, proving the stability of this heterogeneous system. The encapsulation of metallo-porphyrins in PDMS creates a heterogeneous selective oxidation catalyst that discriminates among molecules on the basis of their mutual affinity. In English EI98014029436 16. Effects of ethanol and other alkanols on transport of acetic acid in Saccharomyces cerevisiae Casal, Margarida (Univ of Minho) Cardoso, Helena | Leao, Cecilia Source: Applied and Environmental Microbiology 64 2 Feb 1998 p 665-668 0099-2240 Abstract: In glucose-grown cells of Saccharomyces cerevisiae IGC 4072, acetic acid enters only by simple diffusion of the undissociated acid. In these cells, ethanol and other alkanols enhanced the passive influx of labelled acetic acid. The influx of the acid followed first-order kinetics with a rate constant that increased exponentially with the alcohol concentration, and an exponential enhancement constant for each alkanol was estimated. The intracellular concentration of labelled acetic acid was also enhanced by alkanols, and the effect increased exponentially with alcohol concentration. Acetic acid is transported across the plasma membrane of acetic acid-, lactic acid-, and ethanol-grown cells by acetate-proton symports. We found that in these cells ethanol and butanol inhibited the transport of labelled acetic acid in a noncompetitive way; the maximum transport velocity decreased with alcohol concentration, while the affinity of the system for acetate was not significantly affected by the alcohol. Semilog plots of Vmax versus alcohol concentration yielded straight lines with negative slopes from which estimates of the inhibition constant for each alkanol could be obtained. The intracellular concentration of labelled acid was significantly reduced in the presence of ethanol or butanol, and the effect increased with the alcohol concentration. We postulate that the absence of an operational carrier for acetate in glucose-grown cells of S. cerevisiae, combined with the relatively high permeability of the plasma membrane for the undissociated acid and the inability of the organism to metabolize acetic acid, could be one of the reasons why this species exhibits low tolerance to acidic environments containing ethanol. In English 17. Functional evaluation of hemoglobin- and lipidheme-vesicles as red cell substitutes Sakai, Hiromi (Waseda Univ) Hamada, Kenichi | Takeoka, Shinji | Nishide, Hiroyuki | Tsuchida, Eishun Source: Polymers for Advanced Technologies 7 8 Aug 1996 John Wiley & Sons Ltd p 639-644 1042-7147 Abstract: The two kinds of red cell substitutes, hemoglobin-vesicles (HbV) and lipidheme-vesicles (LihV, totally synthetic oxygen carrier), were evaluated in terms of physicochemical properties such as binding and dissociating reactions of ligands (CO, O2 and NO), rheological and structural properties. Carbonylation of Hb during the purification of Hb and the preparation of HbV is effective to prevent Hb denaturation. The rates of oxygenation of both HbV and LihV are faster than that of red blood cells (RBC). Their oxygen affinities (P50, HbV, 32 mmHg; LihV, 43 mmHg, cf. RBC, 28 mmHg) can be controlled to transport a sufficient amount of oxygen comparable with that of RBC. The smaller sizes of vesicles are advantageous for prompt ligand reaction and low viscosity. Both HbV and RBC show about 100 times less vasoconstrictive effects than stripped Hb. HbV shows only one sixth of the slow binding rate of NO (= endothelial derived relaxation factor) in comparison with stripped Hb. Inhibition of vasoconstriction by those vesicles is discussed from the kinetic data. In English 39 Refs. EI96103364101

> 안녕하세요. 경희대학교에서 화학공학, 유전공학을 전공하고 있는 노정권이라고 합니다. 제가 특히 알고 싶은 것을 좀 더 특화시킨다면, 특정 단백질을 막분리할 때에 쓰이는 affinity carrier를 만드는 방법입니다. 그 방법에 다른 것보다 antibody가 들어간다면 더욱 도움이 되겠네요. > 주신 자료를 가지고 정말 열심히 공부해서 저도 남들 공부에 도움이 되는 사람이 되도록 하겠습니다. 감사합니다. I found an article..... Theme,name of Journal, Abstract... THE FUNCTIONAL EFFECTS OF BIOTINYLATION OF ANTI-ANGIOTENSIN-CONVERTING ENZYME MONOCLONAL ANTIBODY IN TERMS OF TARGETING IN VIVO Muzykantov V.R.; Gavriluk D.D.; Reinecke A.; Atochina E.N.; Kuo A.; Barnathan E.S.; Fisher A.B. Inst. for Environmental Medicine, Univ. of Pennsylvania, School of Medicine, 36th Street and Hamilton Walk,Philadelphia, PA 19104, USA Abstract The effect of modification with biotin N-hydroxysuccinimide ester of mouse monoclonal antibody to angiotensin-converting enzyme, anti-ACE Mab 9B9, on its targeting to endothelial cells has been studied in vitro and in vivo. By in vitro assay, Mab 9B9 biotinylated at a biotin/IgG molar ratio in reaction mixture (B/IgG ratio) of 0.7-2.2 bound streptavidin monovalently and retained antigen-binding capacity. Mab 9B9 biotinylated at a B/IgG ratio of 20 and higher bound streptavidin polyvalently. Extensive biotinylation (B/IgG ratio of 60 and higher) led to dramatic reduction of Mab 9B9 Ag-binding capacity and to reduction of Mab 9B9 recognition by goat polyclonal antibody to mouse IgG. Radiolabeled Mab 9B9 biotinylated at a B/IgG ratio of 6 (b6-Mab 9B9) bound effectively to cultured vascular endothelium, with affinity characteristics similar to non-biotinylated Mab 9B9. Endothelial cells internalized both Mab 9B9 and b6-Mab 9B9 to the same extent (60% internalization at 3 h incubation at 37°C). Degradation of cell surface-associated Mab 9B9 or b6-Mab 9B9 was very low (< 1% as measured by TCA solubility of radiolabel). In contrast, degradation of internalized b6-Mab 9B9 was more profound than that of Mab 9B9 (20 ± 3% vs 6 ± 1%, P < 0.01). After injection in rats, radiolabeled b6-Mab 9B9 had a biodistribution pattern similar to that of radiolabeled Mab 9B9. Both preparations effectively accumulated in the lung (15-20% of injected dose/g of tissue vs 2% of injected dose/g of blood). Extensive biotinylation led to both a reduction of specific pulmonary uptake of Mab 9B9 and an enhanced blood clearance of Mab 9B9. Streptavidin binding to b6-Mab 9B9 did not alter biodistribution or pulmonary targeting of biotinylated antibody. We conclude that extensive biotinylation induces complex alterations of the functional properties of Mab 9B9. In contrast, b6-Mab 9B9 may serve as an affinity carrier for targeting of biotinylated compounds to the pulmonary endothelium. Index Terms: endothelium cell; endocytosis; drug targeting; monoclonal antibody; biotin derivative; streptavidin

> 안녕하세요. 경희대학교에서 화학공학, 유전공학을 전공하고 있는 노정권이라고 합니다. 제가 특히 알고 싶은 것을 좀 더 특화시킨다면, 특정 단백질을 막분리할 때에 쓰이는 affinity carrier를 만드는 방법입니다. 그 방법에 다른 것보다 antibody가 들어간다면 더욱 도움이 되겠네요. > 주신 자료를 가지고 정말 열심히 공부해서 저도 남들 공부에 도움이 되는 사람이 되도록 하겠습니다. 감사합니다. Here is a Journal article, Keyword: affinity carrier,antibody Theme : Receptor-mediated targeted drug or toxin delivery, Name og Journal :Advanced Drug Delivery Reviews, Volume 29, Issue 3, 2 February 1998, Pages 273-289 Abstract The new approach to the treatment of cancer or to immunomodulation is drug targeting. Cellular uptake of drugs bound to a targeting carrier or to a targetable polymeric carrier is mostly restricted to receptor-mediated endocytosis. Factors that influence the efficiency of receptor-mediated uptake of targeted drug conjugate are the affinity of the targeting moieties, the affinity and nature of the target antigen, density of the target antigen, the epitope of the target antigen, the type of cell target, the rate of endocytosis, the route of internalization of the ligand-receptor complex, the ability of the drug or toxin to release from its targeted carrier, the ability of the drug or toxin to escape from a vesicular compartment into the cytosol, the affinity of the carrier to the drug and the concentration of the carrier. Targeted chemotherapy is also significantly influenced by the antigenic modulation and/or immunoselection of tumor cells. The binding of drug (toxin) to targetable polymeric carrier considerably decreases unwanted side toxicity. Author Keywords: Polymeric carrier; HPMA; Endosomal compartment; Lysosomes; Endocytosis; Targeted chemotherapy; Receptors; Surface antigens; Ligand; Monoclonal antibodies; Side toxicity *Corresponding author. Tel.: +420 2 4752267; fax: +420 2 4721143, +420 2 4715743; e-mail: rihova@biomed.cas.cz

> 안녕하세요. 경희대학교에서 화학공학, 유전공학을 전공하고 있는 노정권이라고 합니다. 제가 특히 알고 싶은 것을 좀 더 특화시킨다면, 특정 단백질을 막분리할 때에 쓰이는 affinity carrier를 만드는 방법입니다. 그 방법에 다른 것보다 antibody가 들어간다면 더욱 도움이 되겠네요. > 주신 자료를 가지고 정말 열심히 공부해서 저도 남들 공부에 도움이 되는 사람이 되도록 하겠습니다. 감사합니다. 다음 사이트는 미국 National Institutes of Science and Technology Web site에 나오는 affinity-membrane separation에 관한 자료입니다. http://www.atp.nist.gov/atp/97wp-seb.htm

> 안녕하세요. 경희대학교에서 화학공학, 유전공학을 전공하고 있는 노정권이라고 합니다. 제가 특히 알고 싶은 것을 좀 더 특화시킨다면, 특정 단백질을 막분리할 때에 쓰이는 affinity carrier를 만드는 방법입니다. 그 방법에 다른 것보다 antibody가 들어간다면 더욱 도움이 되겠네요. > 주신 자료를 가지고 정말 열심히 공부해서 저도 남들 공부에 도움이 되는 사람이 되도록 하겠습니다. 감사합니다. 첨가할 자료입니다. Reference Literature Adisaputro, I., Wu, Y. and Etzel, M. Strong cationic and anionic exchange membranes and beads for protein isolation from whey. J. Liq. Chrom. and Rel. Technol. 19(9), 1437-50 (1996) Belanich, M., Cummings, B., Grob, D., Klein, J., O’Connor, A. and Yarosh, D. Reduction of endotoxin in a protein mixture using strong anion exchange membrane adsorption. Pharmaceutical Technology 20(3), 142-150 (1996) Broverman, S. A. and Prestwich, G.D. Fast Ion-Exchange Membrane Purification of a Microsomal Protein. BioTechniques 19, 874-875 (1995) Champluvier, B., Briefs, G. and Kula, M. R. Affinity (crossflow) microfiltration membranes for isolation of enzymes from crude extracts. Proceedings of the 5th European Congress on Biotechnology. 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Presented at the 4th International Conference on Lactoferrin: Structure, Function and Applications, Sapporo Japan May 1999. Woker, R., Champluvier, B. and Kula, M. R. Purification of S-oxynitrilase from Sorghum bicolor by immobilised metal ion affinity chromatography on different carrier materials. J. Chromatography 584, 85-92 (1992) Zietlow, M. and Etzel, M. Evaluation of sulfopropyl ion-exchange membrane cartridges for isolation of proteins from bovine whey. J. Liquid. Chromatogr., 18 (5), 1001-18 (1995)