KOSEN 한인과학기술자네트워크

Authors Kong L. Li X. Zou HF. Wang HL. Mao XQ. Zhang Q. Ni JY. Title Analysis of terpene compounds in Cimicifuga foetida L. by reversed-phase high-performance liquid chromatography with evaporative light scattering detection Source Journal of Chromatography. A.. 936(1-2):111-118, 2001 Nov 30. Abstract An RP-HPLC method with evaporative light scattering detection (ELSD) was developed for the analysis of terpene compounds in traditional Chinese medicine. Actein, 27-deoxyactein and cimicifugoside in a typical Chinese medicine of Cimicifuga foetida L. were quantitatively analyzed. Comparing ELSD with UV detection under the same eluent conditions, the former showed better sensitivity and a more stable baseline. The ELSD responses versus sample size of three terpenes and those in double logarithmic were investigated. The good calibration curves in double logarithmic coordinator for actein, 27-deoxyactein and cimicifugoside were obtained. Three solutions for the extraction of the terpene compounds were also compared, the results indicated that methanol-water (80:20) is the best among them. The method was applied to quantify actein, 27-deoxyactein and cimicifugoside in Cimicifuga foetida L. from Hunan, China. It was shown that ELSD is an effective detection method for the analysis of the non-volatile terpenes in traditional Chinese medicine. (C) 2001 Elsevier Science B.V. All rights reserved. [References: 21] >triterpene glycoside의 지표물질인 27-deoxyactein의 HPLC를 이용하여 분석하는 방법을 알고 계신분은 도와주세요. > >감사합니다.