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지식나눔

지식나눔

카테킨을 EGCG를 추출하는 과정을 알려주세요

2004-07-01 org.kosen.entty.User@7dc80241 김현중(wave61)
  • 1
녹차에서 카테킨을 추출하는방법과 추출된 카테킨에서 EGCG를 추출,정제하는 방법을 알려주세요.
  • EGCG
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    2004-07-16
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    Authors Amarowicz R. Shahidi F. Wiczkowski W. Title Separation of individual catechins from green tea using silica gel column chromatography and HPLC Source Journal of Food Lipids. 10(2):165-177, 2003 Jul. Abstract Crude catechin mixtures from green tea were separated into six fractions using a silica gel column chromatography and a chloroform-methanol-water (65:35:10, v/v/v, lower phase) solvent system. Fraction I was free of catechins, fraction II contained epicatechin (EC), ftaction III had epicatechin and epigallocatechin (EGC), fraction IV possessed EGC, fraction V contained EGC, epicatechin gallate (ECG) and epigallocatechin gallate (EGCG), and fraction VI had EGCG. EC and EGC were separated from fractions II, III and IV using HPLC with a RP-18 semipreparative column and a water-dimethylformamide-methanol-acetic acid (157:40:2:1, v/v/v/v) solvent system. For isolation of EGC, ECG and EGCG from fractions V and VI a water-acetonitrile-methanol-acetic acid (159:36:4:1, v/v/v/v) solvent system was employed Chemical structures of purified catechins were further confirmed by ESI-MS. [References: 28] >녹차에서 카테킨을 추출하는방법과 추출된 카테킨에서 EGCG를 추출,정제하는 방법을 알려주세요. >
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    Authors Amarowicz R. Shahidi F. Wiczkowski W. Title Separation of individual catechins from green tea using silica gel column chromatography and HPLC Source Journal of Food Lipids. 10(2):165-177, 2003 Jul. Abstract Crude catechin mixtures from green tea were separated into six fractions using a silica gel column chromatography and a chloroform-methanol-water (65:35:10, v/v/v, lower phase) solvent system. Fraction I was free of catechins, fraction II contained epicatechin (EC), ftaction III had epicatechin and epigallocatechin (EGC), fraction IV possessed EGC, fraction V contained EGC, epicatechin gallate (ECG) and epigallocatechin gallate (EGCG), and fraction VI had EGCG. EC and EGC were separated from fractions II, III and IV using HPLC with a RP-18 semipreparative column and a water-dimethylformamide-methanol-acetic acid (157:40:2:1, v/v/v/v) solvent system. For isolation of EGC, ECG and EGCG from fractions V and VI a water-acetonitrile-methanol-acetic acid (159:36:4:1, v/v/v/v) solvent system was employed Chemical structures of purified catechins were further confirmed by ESI-MS. [References: 28] >녹차에서 카테킨을 추출하는방법과 추출된 카테킨에서 EGCG를 추출,정제하는 방법을 알려주세요. >
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