KOSEN 한인과학기술자네트워크

관련된 논문이라고 생각드는 것들 몇개하고 참고가 될 만한 것 서술해 놓았습니다. Screening for osteoporosis in patients with inflammatory bowel disease by using urinary N-telopeptides. European Journal of Gastroenterology & Hepatology. 14(6):599-605, June 2002. ================================================================ Thyroid , April 2003, Vol. 13, No. 4, Pages 347-356 Posted online on July 9, 2004. (doi:10.1089/105072503321669839) Bone Metabolism in Patients with Differentiated Thyroid Carcinoma Receiving Suppressive Levothyroxine Treatment P. Mikosch ================================================================= http://www.sas-centre.org/_new/site/assays/bone_markers/ntelopep.html Analytical information NTx: The assay measures N-telopeptide linked pyridinoline and deoxypyridinoline residues from the breakdown for type 1 collagen in human urine. The antibodies do not recognise non-pyridinoline-linked peptides for free pyridinoline. The assay is a competitive inhibition EIA utilising a monoclonal antibody labelled with horse radish peroxidase. The assay is standardised against a collagenase digested bone collagen and reported in terms of the ratio of bone collagen equivalents (nmol/L) to creatinine concentration (mmol/L). The coefficient of variation for the assay is 7% across the reference range. Biological variation: There is a significant circadian rhythm with higher levels of excretion at night (as much as 50%). This rhythm is the same throughout life, but the form changes in osteoporosis. There is also a day to day variation in excretion of N-telopeptide (quoted as greater than 24%). Patient preparation: I it is recommended that a carefully monitored second void urine collection is made; patients should be asked to empty their bladders on waking and discard the urine. Then they should collect the next specimen (the second morning void) in the container (plain container, no additives). To minimise the day to day variability in serial measurement it is important to maintain the same collection protocol. Stability: The NTx levels in urine are reasonably stable; it is recommended that urines be stored at 2-8C for up to 7 days, but deep frozen at -20C if longer storage is expected. It is recommended that a sample stored at -20C should not be subjected to more than 5 freeze/that cycles. Furthermore, it is recommended that samples should not be stored at any stage in direct sunlight. It is recommended that samples should be collected without preservative and therefore storage at 2-8C is preferred to avoid contamination. There is data to suggest that NTx levels are more variable where samples are collected with a preservative possibly due to precipitation of other components in urine at 2-8C. There are differences between the laboratories in reference ranges, call and check for latest reference ranges. ==================================================================== http://www.amc.seoul.kr/~amccp/menu2/chemistry/L3636.html N-telopeptide L3636 1. 검사원리 Osteomark는 NTx가 흡착된 Microwell을 사용한 competitive-inhibition ELISA이다. Horseradish peroxidase로 결합된 Monoclonal antibody가 환자 검체의 NTx와 Microwell에 흡착된 NTx를 경쟁적으로 결합한다. Microwell에 결합된 antibody의 양은 검사물의 NTx농도와 반비례하게 되며, 그 농도 값은 광학적으로 측정하여 standard calibration curve에서 계산한다. >N-telopeptide가 무엇인지요? >골다공증을 진단하는 marker라고 하는데 >자세히 설명 부탁 드립니다. >가능하면 논문도요~~

jhjeon2님이 올려주신 파일이 페이지가 안 열리던데 수고스럽겠지만 다시 올려주시면 안될까여? 부탁드립니다

다시 올립니다. 만약에 또 안되면 메일로 보내드릴께요... >N-telopeptide가 무엇인지요? >골다공증을 진단하는 marker라고 하는데 >자세히 설명 부탁 드립니다. >가능하면 논문도요~~

현재 임상적 유용성이 밝혀진 bone metabolism의 생화학적 지표물질로는 Bone formation에 관여하는 Serum bone-specific alkaline phosphatase, Serum osteocalcin, Serum propeptide of type I procollagen과 Bone resorption에 관여하는 Urine and serum cross-linked N-telopeptide, Urine and serum cross-linked C-telopeptide, Urine total free deoxypyridinoline, Urine hydroxyproline, Serum tartrate-resistant acid phosphatase, Serum bone sialoprotein 및 Urine hydroxylysine glycosides 등이 알려져 있습니다. NTx는 (Cross-linked N-teleopeptide of type I collagen)은 Bone resorption marker로 urine이나 serum을 이용하여 ELISA format이나 Point-of-Care (POC) device를 이용하여 쉽게 측정할 수 있습니다. Osteomark NTx point-of-care device의 경우 골다공증 치료 시작 후 의사의 지시하에 bone resorption 변화를 monitoring 위한 home use 방법으로 FDA의 승인얻었습니다. NTx와 그에 대한 측정 방법은 www.ostex.com에 가시면 좀 더 많은 정보를 얻을 수 있습니다. >N-telopeptide가 무엇인지요? >골다공증을 진단하는 marker라고 하는데 >자세히 설명 부탁 드립니다. >가능하면 논문도요~~