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What is GST full down assay?

2006-06-12 org.kosen.entty.User@61008a13 신동훈(gabriel78)
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What is GST full down assay?
  • GST full down assay
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    정철웅님의 답변

    2006-06-13
    • 0
    >What is GST full down assay? 간단하게 답하자면, protein interaction을 보기위해서 사용하는 방법 중 하나입니다. 보통 antibody를 이용한 immunoprecipitation을 최우선으로 하지만 antibody가 없을 경우 GST-fusion protein 을 만든후 bead를 이용해 분리해 내는 것이죠
    답변수정
    >What is GST full down assay? 간단하게 답하자면, protein interaction을 보기위해서 사용하는 방법 중 하나입니다. 보통 antibody를 이용한 immunoprecipitation을 최우선으로 하지만 antibody가 없을 경우 GST-fusion protein 을 만든후 bead를 이용해 분리해 내는 것이죠
    등록된 댓글이 없습니다.
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    배우철님의 답변

    2006-06-13
    • 0
    GST pull down assay가 아닌가요?? GST pull-down assay is a simple technique to test interaction between a tagged protein or the bait (GST, His6, biotin ...) and another protein (test protein, or prey). The bait protein, purified from an appropriate expression system (e.g., Escherichia coli), is immobilized on a glutathione affinity gel. The bait serves as the secondary affinity support for identifying new protein partners or for confirming a previously suspected protein partner to the bait. Prey protein can be obtained from multiple sources including recombinant purified proteins, cell lysate or in vitro transcription/translation reactions. Protein-protein interactions can be visualized by SDS-PAGE and associated detection methods depending on the sensitivity requirements of the interacting proteins. These methods include Coomassie or silver staining, Western blotting and [35S] radioisotopic detection. 세부 실험정보는 아래 화일을 참조하세요.. www.imbv.uio.no/cel/groups/bakkegroup/ db01-news/internet/html/docs/4.4.pdf >What is GST full down assay?
    답변수정
    GST pull down assay가 아닌가요?? GST pull-down assay is a simple technique to test interaction between a tagged protein or the bait (GST, His6, biotin ...) and another protein (test protein, or prey). The bait protein, purified from an appropriate expression system (e.g., Escherichia coli), is immobilized on a glutathione affinity gel. The bait serves as the secondary affinity support for identifying new protein partners or for confirming a previously suspected protein partner to the bait. Prey protein can be obtained from multiple sources including recombinant purified proteins, cell lysate or in vitro transcription/translation reactions. Protein-protein interactions can be visualized by SDS-PAGE and associated detection methods depending on the sensitivity requirements of the interacting proteins. These methods include Coomassie or silver staining, Western blotting and [35S] radioisotopic detection. 세부 실험정보는 아래 화일을 참조하세요.. www.imbv.uio.no/cel/groups/bakkegroup/ db01-news/internet/html/docs/4.4.pdf >What is GST full down assay?
    등록된 댓글이 없습니다.
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