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지식나눔

지식나눔

chromosome walking

2006-06-20 org.kosen.entty.User@45db755b 최동규(cdkcbk)
  • 1
수업인데..자세히 좀 가르쳐 주세요^^
  • chromosome
지식의 출발은 질문, 모든 지식의 완성은 답변! 
각 분야 한인연구자와 현업 전문가분들의 답변을 기다립니다.
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    배우철님의 답변

    2006-06-20
    • 0
    Chromosome walking은 염색체 이동이라고 하는데요.. 일련의 중복된 제한효소의 처리로 단편들을 확인하기 위해 이용되는 기술입니다. 흔히 큰 DNA분자 위에서 유전자들의 상대적인 위치를 결정하는데 이용되고 있습니다. Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome. As the traditional chain termination method does not allow long DNA strands to be sequenced, this method works by dividing the long sequence into several consecutive short ones. The basic technique is as follows: 1. A primer that matches the beginning of the DNA to sequence is used to synthesize a short DNA strand complementary to the unknown sequence, starting with the primer (see PCR). 2. The new short DNA strand is sequenced using the chain termination method. 3. The end of the sequenced strand is used as a primer for the next part of the long DNA sequence. That way, the short part of the long DNA that is sequenced keeps “walking“ along the sequence. The method can be used to sequence entire chromosomes (thus, chromosome walking). A different method with the same purpose which becomes more popular for large-scale sequencing >수업인데..자세히 좀 가르쳐 주세요^^
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    Chromosome walking은 염색체 이동이라고 하는데요.. 일련의 중복된 제한효소의 처리로 단편들을 확인하기 위해 이용되는 기술입니다. 흔히 큰 DNA분자 위에서 유전자들의 상대적인 위치를 결정하는데 이용되고 있습니다. Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome. As the traditional chain termination method does not allow long DNA strands to be sequenced, this method works by dividing the long sequence into several consecutive short ones. The basic technique is as follows: 1. A primer that matches the beginning of the DNA to sequence is used to synthesize a short DNA strand complementary to the unknown sequence, starting with the primer (see PCR). 2. The new short DNA strand is sequenced using the chain termination method. 3. The end of the sequenced strand is used as a primer for the next part of the long DNA sequence. That way, the short part of the long DNA that is sequenced keeps “walking“ along the sequence. The method can be used to sequence entire chromosomes (thus, chromosome walking). A different method with the same purpose which becomes more popular for large-scale sequencing >수업인데..자세히 좀 가르쳐 주세요^^
    등록된 댓글이 없습니다.
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