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지식나눔

지식나눔

MOI에 대해서 좀 가르쳐 주십시오

2007-09-06 org.kosen.entty.User@41044e85 최훈성(hschoi77)
  • 1
MOI로 virus titer를 어떻게 표현할지 궁금하네요 figure와 regend를 쓸때 형식좀 더불어서 가르쳐주시면 고맙겠습니다.
  • MOI
  • virus
지식의 출발은 질문, 모든 지식의 완성은 답변! 
각 분야 한인연구자와 현업 전문가분들의 답변을 기다립니다.
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    장성재님의 답변

    2007-09-06
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    MOI(Multiplicity of Infection)란 한개의 세포에 감염된 바이러스의 수를 말합니다. 표적 세포에 대한 “바이러스 투여량 (viral dose)“으로 사용되기도 한답니다. 예를 들어, 1-5의 MOI 라 함은 표적 세포에 1-5개의 바이러스 입자가 감염된다고 보면 됩니다. 일반적으로 titer를 mL당 IU (Infectious unit)으로 표시하지만, 논문 등에서는 IU/mL과 더불어 MOI를 동시에 표시하기도 합니다. 밑의 예시에서 보면 CSCG (virus titer, 0.3 × 10^6 IU/ml; MOI, 0.75)처럼 말입니다. Figure regend는 관련 저널의 규정에 의거해서 작성하는 것이 좋습니다. 밑에 Journal of Virology에 실린 Figure regend를 예시합니다. CS-Rh-MLV-E vector allows a high level of EGFP expression in primary activated T cells. (A) Flow cytometry analysis of CSCG-, CS-MLV-E-, and CS-Rh-MLV-E-infected T cells on day 3 or day 8 postinfection. (B) EGFP expressions in the infected CD4+ and CD8+ T-cell subsets were similar. Human PBMC were activated by plate-bound anti-CD3 and anti-CD28 MAbs for 60 h. Activated T-cell blasts were infected by virus supernatant of CSCG (virus titer, 0.3 × 106 IU/ml; MOI, 0.75), CS-MLV-E (virus titer, 1.1 × 106 IU/ml; MOI, 0.55), CS-MLV-E (virus titer, 1.3 × 106 IU/ml; MOI, 0.65), CS-Rh-MLV-E (virus titer, 1.9 × 106 IU/ml; MOI, 0.48), and CS-Rh-MLV-E (virus titer, 1.2 × 106 IU/ml; MOI, 0.3). The percentage of EGFP+ cells and the MFI of EGFP expression of the infected cells were analyzed by flow cytometry on days 3 and 8 postinfection. On day 8, aliquots of the infected cells were also stained for surface expression of CD4 and CD8 molecules and analyzed by fluorescence-activated cell sorter. (C) Flow cytometry analysis of the CSCG-, CS-RhMLV-E-, and SRLEGFP-infected SUPT1 and activated primary T cells. SUPT1 cells were infected by virus supernatant of CSCG (virus titer, 1 × 106 IU/ml; MOI, 2.5), CS-RhMLV-E (virus titer, 1.4 × 106 IU/ml; MOI, 0.07), and SRLEGFP (virus titer, 7 × 104 IU/ml; MOI, 0.18) at the following dilutions: undiluted (1×) for CSCG, 1/50× dilution for CS-Rh-MLV-E, and 1× for SRLEGFP. Activated T-cell blasts were infected by undiluted virus supernatant of CSCG (virus titer, 1 × 106 IU/ml; MOI, 2.5) and CS-RhMLV-E (virus titer, 1.4 × 106 IU/ml; MOI, 3.5), except for SRLEGFP, where 140×-concentrated virus supernatant (virus titer, 9.8 × 106 IU/ml; MOI, 25) was used in the infection. The percentage of EGFP+ cells and the MFI of EGFP expression of the infected cells were analyzed by flow cytometry on day 3 postinfection. FSC, forward scatter; FL, fluorescence. 출처: Journal of Virology, April 2000, p. 3668-3681, Vol. 74, No. 8
    답변수정
    MOI(Multiplicity of Infection)란 한개의 세포에 감염된 바이러스의 수를 말합니다. 표적 세포에 대한 “바이러스 투여량 (viral dose)“으로 사용되기도 한답니다. 예를 들어, 1-5의 MOI 라 함은 표적 세포에 1-5개의 바이러스 입자가 감염된다고 보면 됩니다. 일반적으로 titer를 mL당 IU (Infectious unit)으로 표시하지만, 논문 등에서는 IU/mL과 더불어 MOI를 동시에 표시하기도 합니다. 밑의 예시에서 보면 CSCG (virus titer, 0.3 × 10^6 IU/ml; MOI, 0.75)처럼 말입니다. Figure regend는 관련 저널의 규정에 의거해서 작성하는 것이 좋습니다. 밑에 Journal of Virology에 실린 Figure regend를 예시합니다. CS-Rh-MLV-E vector allows a high level of EGFP expression in primary activated T cells. (A) Flow cytometry analysis of CSCG-, CS-MLV-E-, and CS-Rh-MLV-E-infected T cells on day 3 or day 8 postinfection. (B) EGFP expressions in the infected CD4+ and CD8+ T-cell subsets were similar. Human PBMC were activated by plate-bound anti-CD3 and anti-CD28 MAbs for 60 h. Activated T-cell blasts were infected by virus supernatant of CSCG (virus titer, 0.3 × 106 IU/ml; MOI, 0.75), CS-MLV-E (virus titer, 1.1 × 106 IU/ml; MOI, 0.55), CS-MLV-E (virus titer, 1.3 × 106 IU/ml; MOI, 0.65), CS-Rh-MLV-E (virus titer, 1.9 × 106 IU/ml; MOI, 0.48), and CS-Rh-MLV-E (virus titer, 1.2 × 106 IU/ml; MOI, 0.3). The percentage of EGFP+ cells and the MFI of EGFP expression of the infected cells were analyzed by flow cytometry on days 3 and 8 postinfection. On day 8, aliquots of the infected cells were also stained for surface expression of CD4 and CD8 molecules and analyzed by fluorescence-activated cell sorter. (C) Flow cytometry analysis of the CSCG-, CS-RhMLV-E-, and SRLEGFP-infected SUPT1 and activated primary T cells. SUPT1 cells were infected by virus supernatant of CSCG (virus titer, 1 × 106 IU/ml; MOI, 2.5), CS-RhMLV-E (virus titer, 1.4 × 106 IU/ml; MOI, 0.07), and SRLEGFP (virus titer, 7 × 104 IU/ml; MOI, 0.18) at the following dilutions: undiluted (1×) for CSCG, 1/50× dilution for CS-Rh-MLV-E, and 1× for SRLEGFP. Activated T-cell blasts were infected by undiluted virus supernatant of CSCG (virus titer, 1 × 106 IU/ml; MOI, 2.5) and CS-RhMLV-E (virus titer, 1.4 × 106 IU/ml; MOI, 3.5), except for SRLEGFP, where 140×-concentrated virus supernatant (virus titer, 9.8 × 106 IU/ml; MOI, 25) was used in the infection. The percentage of EGFP+ cells and the MFI of EGFP expression of the infected cells were analyzed by flow cytometry on day 3 postinfection. FSC, forward scatter; FL, fluorescence. 출처: Journal of Virology, April 2000, p. 3668-3681, Vol. 74, No. 8
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