KOSEN 한인과학기술자네트워크

항체를 이용해서 직접 ELISA법으로 측정하실 수 있을 것 같습니다만... 참고로 논문에서 발췌한 내용을 아래에 첨부합니다. S-100 Protein Analysis The concentration of S-100 protein in blood samples was measured as described earlier.11 In brief, all measurements were set up in duplicate. Microtiter plates coated with 10 µL of anti–S-100 ß chain (Sigma) in 20 mL phosphate buffer (0.05 mol/L, pH 8.6) were incubated with 200 µL per well of S-100 calibrators, controls, and samples for 120 minutes. Biotin-labeled rabbit anti–S-100 antibody (DAKO) in a Tris 0.05 mol/L, NaCl 0.15 mol/L, CaCl2 10 mmol/L, NaN3 0.15 mmol/L buffer was added, and plates were incubated for another hour. After the plates had been washed, 200 µL of streptavidin-europium in assay buffer (Tris 0.05 mol/L, NaCl 0.15 mol/L, bovine serum albumin 1 g/L, bovine gamma globulin 0.5 g/L, both from Sigma, NaN3 0.15 mmol/L) was added to each well, and the plates were incubated for 30 minutes. As a last step, 200 µL of enhancement solution (acetic acid 0.01 mol/L, tri-n-octyl phosphine oxide 38 mg/L, potassium phtalate 1.3 g/L, thenoyltrifluoroacetone 222 mg/L, Triton X-100 2 mL/L) was added to each well, and the plates were incubated for 15 minutes. The resulting fluorescence was measured with a DELFIA 1232 fluorometer (Wallac). reference S-100 Protein and Neuron-Specific Enolase Concentrations in Blood as Indicators of Infarction Volume and Prognosis in Acute Ischemic Stroke. Stroke. 1997;28:1956-1960. >brain injury후에 serum과 CSF에서 증가한다고 알려진 S-100 serum농도를 측정할 수 있는 병원이 한국에 있나여? >있다면 어느 병원에서 할 수 있죠?