KOSEN 한인과학기술자네트워크

보고 있는 논문인데 처음이라 많이 부족합니다. 그림에 대한 설명 좀 부탁 드리빈다. 미생물쪽엔 처음인데.. ㅠㅠ 많이 힘드네요... 자세한 설명 부탁 드립니다. ?Figure 1 | Comparison of data sets obtained from the caecal microbiomes of obese and lean littermates. a, Number of observed orthologous groups in each caecal microbiome. Black indicates the number of observed groups. ?Grey indicates the number of predicted missed groups. b, Relative abundance of a subset of COG categories (BLASTX, e-value,1025) in the lean1 (red) and ob1 (blue) caecal microbiome, characterized by capillaryand pyro-sequencers (squares and triangles, respectively). c, d, A subset of COG categories (c) and all KEGG pathways (d) consistently enriched or depleted in the caecal microbiomes of both obese mice compared with their lean littermates. Red denotes enrichment and green indicates depletion on the basis of a cumulative binomial test (brightness indicates the level of significance). Black indicates pathways whose representation is not significantly different. Asterisks indicate groups that were consistently enriched or depleted between both sibling pairs using a more stringent EGT assignment strategy (e-value,1028). For additional details see Supplementary Discussion; Supplementary Figs 5 and 6, and Supplementary Tables 6, 8 and 9. ?a, Clustering of caecal microbiomes of obese and lean sibling pairs based on reciprocal TBLASTX comparisons. All possible reciprocal TBLASTX comparisons of microbiomes (defined by capillary sequencing) were performed from both lean and obese sibling pairs. A distance matrix was then created using the cumulative bitscore for each comparison and the cumulative score for each self?self comparison. Microbiomes were subsequently clustered using NEIGHBOUR (PHYLIP version 3.64). b, Principal Component Analysis (PCA) of KEGG pathway assignments. A matrix was constructed containing the number of EGTs assigned to each KEGG pathway in each microbiome (includes KEGG pathways with >0.6% relative abundance in at least two microbiomes, and a standard deviation >0.3 across all microbiomes), PCA was performed using Cluster3.0 (ref. 1 25), and the results graphed along the first two components. ?a, Gas-chromatography mass-spectrometry quantification of short-chain fatty acids in the caeca of lean (n = 4) and obese (n = 5) conventionally raised C57BL/6J mice. b, Bomb calorimetry of the faecal gross energy content (kcal g-1) of lean (+/+, ob/+; n = 9) and obese (ob/ob; n = 13) conventionally raised C57BL/6J mice. c, Colonization of germ-free wild-type C57BL/6J mice with a caecal microbiota harvested from obese donors (ob/ob; n = 9 recipients) results in a significantly greater percentage increase in total body fat than colonization with a microbiota from lean donors (+/+; n = 10 recipients). Total body fat content was measured before and after a two-week colonization, using dual-energy X-ray absorptiometry. Mean values s.e.m. are plotted. Asterisks indicate significant differences (two-tailed Student's t-test of all datapoints, *P < 0.05, **P 0.01, ***P < 0.001).