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지식나눔

지식나눔

Xanthine oxidase 실험관련 질문입니다.

2013-04-29 org.kosen.entty.User@3c1ee03 이대훈(ldhoonlb)
  • 1
Xanthine oxidase 저해활성 실험관련 질문입니다. 실험 진행 중 Xanthine과 Xanthine oxidase효소를 농도별로 반응 시켜 보아도 반응이 되질 않습니다. 그런데, Xanthine 시약(기질)을 녹인 뒤 며칠 지나니 뿌연색 가루가 많이 가라 않아 있습니다. 혹시 Xanthine시약 녹이는법에 대해 알고 싶습니다..^^
  • Xanthine oxidase
  • Xanthine
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    강광철님의 답변

    2013-04-29
    • 0
    검색해보니 알칼리 용액에 녹는다고 하네요. Most xanthine derivatives dissolve in alkaline solutions - either water (if you are lucky) or methanol. Try dissolving it as best you can and then add a minimal amount of eg. 1 M NaOH until it dissolves completely. Hopefully you will not be adding enough of this to affect the final pH of your culture medium. Yep, just had a look at my ancient copy of the Merck Index and found that xanthine has a maximal solubility in water of < 0.5 mM and you are trying for ca. 66 mM. Merck says that xanthine is freely soluble in NaOH solutions and also in mineral acids so you might also try pushing the pH in the acid direction. 참고하세요..
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    검색해보니 알칼리 용액에 녹는다고 하네요. Most xanthine derivatives dissolve in alkaline solutions - either water (if you are lucky) or methanol. Try dissolving it as best you can and then add a minimal amount of eg. 1 M NaOH until it dissolves completely. Hopefully you will not be adding enough of this to affect the final pH of your culture medium. Yep, just had a look at my ancient copy of the Merck Index and found that xanthine has a maximal solubility in water of < 0.5 mM and you are trying for ca. 66 mM. Merck says that xanthine is freely soluble in NaOH solutions and also in mineral acids so you might also try pushing the pH in the acid direction. 참고하세요..
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