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지식나눔

지식나눔

Hydrocortisone 희석 문의드립니다

2014-02-04 org.kosen.entty.User@439955ef 박선욱(glad8888)
  • 2
.
  • H0135 Sigma
  • Hydrocortisone
  • 농도
지식의 출발은 질문, 모든 지식의 완성은 답변! 
각 분야 한인연구자와 현업 전문가분들의 답변을 기다립니다.
답변하기
답변 2
  • 답변

    신동협님의 답변

    2014-02-04
    • 2

    첨부파일

    http://www4.mpbio.com/ecom/docs/proddata.nsf/(webtds2)/194568

    Solubility: Soluble (mg/ml) at 25oC in: water 0.28; ethanol 15.0; methanol 6.2; acetone 9.3; chloroform 1.6; propylene glycol 12.7; ether ~ 0.35. Soluble in concentrated sulfuric acid with intense green fluorescence.1

    For the water soluble hydrocortisone (cat#: 199339): Typical stock solutions can be prepared at concentrations of 50-500 mg solid (total powder) in 1 ml water (difficulty may be encountered when attempting to solubilize in salt solutions or buffers). Solutions can be sterile filtered using a 0.2 um membrane. Solutions should be stored in working aliquots at -20°C. When calculating molar solutions, the molecular weight of the cyclodextrin component of the complex is typically disregarded and the solution is prepared considering the amount of the hydrocortisone in the preparation (100 mg/gram solid) and its respective molecular weight.
     
    답변수정
    http://www4.mpbio.com/ecom/docs/proddata.nsf/(webtds2)/194568

    Solubility: Soluble (mg/ml) at 25oC in: water 0.28; ethanol 15.0; methanol 6.2; acetone 9.3; chloroform 1.6; propylene glycol 12.7; ether ~ 0.35. Soluble in concentrated sulfuric acid with intense green fluorescence.1

    For the water soluble hydrocortisone (cat#: 199339): Typical stock solutions can be prepared at concentrations of 50-500 mg solid (total powder) in 1 ml water (difficulty may be encountered when attempting to solubilize in salt solutions or buffers). Solutions can be sterile filtered using a 0.2 um membrane. Solutions should be stored in working aliquots at -20°C. When calculating molar solutions, the molecular weight of the cyclodextrin component of the complex is typically disregarded and the solution is prepared considering the amount of the hydrocortisone in the preparation (100 mg/gram solid) and its respective molecular weight.
     
    박선욱(glad8888) 2014-03-18

    ？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？??？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？ ？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？

  • 답변

    이배훈님의 답변

    2014-02-04
    • 2

    첨부파일



    H 0135 HYDROCORTISONE 1 mg  용량 ---- 상온 보관 (고체 파우더)

    To prepare 50 µg/ml stock solution   ---stock 제조
     
    add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.
    (100% 에탄올 1ml을 1mg powder 용기에 넣고 녹인후 (gentle 하게 mixing) 19ml 멸균
    media에 추가 (1mg/20ml: 50ug/ml stock solution)---aliquots (1-2ml 용기에 ) 냉동 보관

    필요할 때 1개씩 꺼내 사용


    0.004-5 µg/ml -working solution (약 50ng/ml)

    1ml stock solution+999ml media  만들어 사용하면 됩니다.

     
    답변수정


    H 0135 HYDROCORTISONE 1 mg  용량 ---- 상온 보관 (고체 파우더)

    To prepare 50 µg/ml stock solution   ---stock 제조
     
    add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.
    (100% 에탄올 1ml을 1mg powder 용기에 넣고 녹인후 (gentle 하게 mixing) 19ml 멸균
    media에 추가 (1mg/20ml: 50ug/ml stock solution)---aliquots (1-2ml 용기에 ) 냉동 보관

    필요할 때 1개씩 꺼내 사용


    0.004-5 µg/ml -working solution (약 50ng/ml)

    1ml stock solution+999ml media  만들어 사용하면 됩니다.

     
    박선욱(glad8888) 2014-04-20

    ？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？??？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？ ？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？

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