2014-03-19
org.kosen.entty.User@24bb1334
박영환(youngh11)
- 2
Purified BMDC were further seeded at 1 x 106/ml/well in 24-well plates with
GM-CSF-supplemented medium as usual; then, 1 ug/ml LPS was added to the
culture at various time points: Immediately (0 h), 2 h, 5 h, 10 h, or 22 h later,
supernatant was collected from each well 22 h after the administration of LPS
for cytokine measurement. Medium alone-treated DC were used as controls.
Purified BMDC stimulated immediately with LPS after isolation (time 0 h) in
culture were termed eDC, and BMDC cultured in medium alone for specific
time periods (2 h) after isolation prior to the addition of LPS were
termed lDC.
특히 붉은글씨부분을 정확하게 해석좀 부탁드릴게요..
GM-CSF-supplemented medium as usual; then, 1 ug/ml LPS was added to the
culture at various time points: Immediately (0 h), 2 h, 5 h, 10 h, or 22 h later,
supernatant was collected from each well 22 h after the administration of LPS
for cytokine measurement. Medium alone-treated DC were used as controls.
Purified BMDC stimulated immediately with LPS after isolation (time 0 h) in
culture were termed eDC, and BMDC cultured in medium alone for specific
time periods (2 h) after isolation prior to the addition of LPS were
termed lDC.
특히 붉은글씨부분을 정확하게 해석좀 부탁드릴게요..
- Dendritic cell
- BMDC
- LPS
지식의 출발은 질문, 모든 지식의 완성은 답변!
각 분야 한인연구자와 현업 전문가분들의 답변을 기다립니다.
각 분야 한인연구자와 현업 전문가분들의 답변을 기다립니다.
답변 2
-
답변
이배훈님의 답변
2014-03-19- 0
첨부파일
Purified BMDC were further seeded at 1 x 106/ml/well in 24-well plates with
GM-CSF-supplemented medium as usual; then, 1 ug/ml LPS was added to the
culture at various time points: Immediately (0 h), 2 h, 5 h, 10 h, or 22 h later,
supernatant was collected from each well 22 h after the administration of LPS
for cytokine measurement.
정제된 BMDC를 24 well 플레이트에 well 당 1 x 106/ml 농도로 보통과 같이 GM-CSF로 보충된 배양액으로 seeding을 하였고, 그 때 1 ug/ml LPS를 다양한 시간 때에 배양액에 첨가하였는데 첨가한 시간때는 seeding후 즉시(o시간), 2 시간, 5시간, 10시간, 22 시간 후이며, 사이토카인 측정을 위해 LPS 첨가(투여) 후(의미상 모든 LPS 투여후(첨가후)) 22 시간 뒤 에 각 well로 부터 상등액을 채취하였다. -
답변
윤상진님의 답변
2018-02-27- 0
Immediately (0 h), 2 h, 5 h, 10 h, or 22 h later,
supernatant was collected from each well 22 h after the administration of LPS
for cytokine measurement.
즉시 (0 시간), 2 시간, 5 시간, 10 시간 또는 22 시간 후에, 사이토 카인 측정을 위해 LPS 투여 22 시간 후 각 웰로부터 상등액을 수집 하였다.