KOSEN 한인과학기술자네트워크

Cap-independent translation of eukaryotic mRNAs requires a specific RNA structure, named internal ribosomal entry site(IRES), and IRES-specific cellular factors along with basic translational machinery. We are investigating molecular mechanism of translation via IRESs by revealing the roles of cis-acting elements in various IRES [viral (encephalomyocarditis virus, poliovirus and hepatitis C virus) and celluar (BiP and c-myc) IRESs] and celluar factors (ITAFs) specifically enhancing the IRES function. We are trying to reveal the moecular details of translational regulation of gene expression at virous physiological conditions such as inflammation, heat stress, and viral infection. In this respect, translational regulation by inflammatory signaling molecules such as prostaglandins has been investigated. We also study the molecular mechanism of translational repression by siRNAs and that of persistent translation of stress resistant mRNAs is repressed. HCV is a pathogenic virus causing hepatitis, liver cirrhosis, and hepatocellular carcinoma. More than 170 million people are suffering from this virus worldwide. We are investigating the moecular basis of the pathogenic processes of HCV. We showed that a human protein GRP94 is required for viral proliferation and that GRP94 is involved in persistent production of HCV in the presence of a cytokine(TRAIL) triggering host cell death. Currently we are developing a cure for HCV infection based on the inactivation of GRP94. APtamers are polynucleotides specifically interacting with target proteins. We are trying to improve aptamer technology and make it more useful in reseasrches, diagnostics, and therapeutics. In collaboration with other laboratories, we are developing processes of utilizing aptamers in immunopreciptitation-type of research, real time visualization of proteins in cells, ELISA-type of diagnostics, and therapeutics by generating agonistic and antagonistic aptamers.