전체 8345
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- Modeling Cancer in 3 Dimensions (NIH-Only)
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- - Brugge, Joan.
National Cancer Institute (U.S.) (2010/03/04) - - Category : NCI CCR Grand Rounds (NIH Only)
- - Brugge, Joan.
Modeling Cancer in 3 Dimensions (NIH-Only)
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- NIH Extramural Administrative Officer Monthly Meeting - April 2007 (NIH Only)
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- - NIH (2010/03/04)
- - Category : Administrative Officer Meetings (NIH Only)
- NIH Extramural Administrative Officer Meeting
NIH Extramural Administrative Officer Monthly Meeting - April 2007 (NIH Only)
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- Demystifying Medicine - ATP Binding Cassette Proteins: Expanding Scope of their Diseases
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- - Gottesman, Michael M.
National Institutes of Health (U.S.) (2010/03/04) - - Category : Demystifying Medicine
- - Gottesman, Michael M.
Demystifying Medicine - ATP Binding Cassette Proteins: Expanding Scope of their Diseases
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- Synaptic Plasticity: Multiple Mechanisms and Functions
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- - Malenka, Robert C.
National Institutes of Health (U.S.) (2010/03/04) - - Category : Neuroscience
- - Malenka, Robert C.
Synaptic Plasticity: Multiple Mechanisms and Functions
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- Developing a Meaningful Relationship with your Teenager
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- - The NIH Work Life Center (WLC) (2010/03/04)
- - Category : Work/Life Center
For more information, visit
http://wlc.od.nih.gov
Developing a Meaningful Relationship with your Teenager
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- Wildlife Surveillance: Early Warning System for Ebola, Avian Flu and other Disease Outbreaks
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- - Karesh, Billy.
National Institutes of Health (U.S.). Emergency Preparedness and Biodefense Interest Group. (2010/03/04) - - Category : Emergency Preparedness and Biodefense
- - Karesh, Billy.
Wildlife Surveillance: Early Warning System for Ebola, Avian Flu and other Disease Outbreaks
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- RNAi and Development in C Elegans
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- - Craig C. Mello, Ph.D., Nobel Prize recipient, University of Massachusetts Medical School (2010/03/04)
- - Category : Wednesday Afternoon Lectures
- Special Thursday NIH Director's Lecture
Argonaute proteins interact with small RNAs to mediate gene silencing. C. elegans contains 27 Argonaute homologs, raising the question of what roles these genes play in RNAi and related gene-silencing pathways. Through our collaborator, Dr. Shohei Mitani, we have obtained a set of 30 deletion alleles representing all of the previously uncharacterized Argonaute genes. Analysis of single- and multiple-Argonaute mutant strains reveals essential functions in several pathways including: (i) chromosome segregation, (ii) fertility, and (iii) at least two separate steps in the RNAi pathway. We show that RDE-1 interacts with trigger-derived sense and antisense RNAs to initiate RNAi, while several other Argonaute proteins interact with amplified antisense siRNAs to mediate downstream silencing. Overexpression of downstream Argonautes enhances silencing, suggesting that these proteins are limiting for RNAi. These downstream Argonautes also function in endogenous RNAi (endo-RNAi) pathways of unknown function. A distinct Argonaute, ERGO-1, appears to function in a manner analogous to RDE-1 at an upstream step in the endo-RNAi pathway. The ERGO-1 and RDE-1 mediated pathways appear to compete for the downstream secondary Argonautes which lack key residues required for mRNA cleavage. Thus our findings support a two-step model for RNAi, in which Argonaute proteins function sequentially, and downstream silencing is mediated by a set of Argonautes unlikely to harbor catalytic-slicer activity.
Nobel Prize recipient Craig C. Mello, PhD, is the Blais University Chair in Molecular Medicine at the University of Massachusetts Medical School (UMMS). He was also designated an Investigator of the Howard Hughes Medical Institute in 2000, the third HHMI researcher selected at UMMS. HHMI is a $13 billion medical research organization that employs more than 350 eminent researchers at 72 medical schools, universities and research institutes worldwide. Dr. Mello holds his BS in biochemistry from Brown University and his PhD in Cellular and Developmental Biology from Harvard University. He was a postdoctoral fellow at the Fred Hutchinson Cancer Research Center before coming to UMMS in 1995. He is also a 1995 Pew Scholar in the Biomedical Sciences. Mello and his colleague Andrew Fire, PhD, formerly of the Carnegie Institution of Washington, received the 2006 Nobel Prize in Medicine for their discovery of RNA interference (RNAi). They demonstrated that a certain form of RNA had the unanticipated property of silencing—or interfering with—the expression of a gene whose coding sequence of DNA was similar to that of the RNA they tested. The RNAi mechanism—a natural response of an organism to double-stranded RNA, of which many viruses are comprised—destroys the gene products that a virus needs to replicate itself, essentially halting the progression of the invading viral infection.
The discovery, which offers astounding potential for understanding and manipulating the cellular basis of human disease, has had two extraordinary impacts on biological science. One is as a research tool: RNAi is now the state-of-the-art method by which scientists can knock down the expression of specific genes in cells, to thus define the biological functions of those genes. But just as important has been the finding that RNA interference is a normal process of genetic regulation that takes place during development. Thus, RNAi has provided not only a powerful research tool for experimentally knocking out the expression of specific genes, but has opened a completely new and totally unanticipated window on developmental gene regulation.
WALS
RNAi and Development in C Elegans
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- Embryonic Stem Cells in Disease and Development
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- - Daley, George.
National Institutes of Health (U.S.). Stem Cell Interest Group. (2010/03/04) - - Category : Stem Cell
- - Daley, George.
Embryonic Stem Cells in Disease and Development
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- Arthropod-Borne Diseases in the Lymelight
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- - Fikrig, Erol.
National Institutes of Health (U.S.). Immunology Interest Group. (2010/03/04) - - Category : Immunology
- - Fikrig, Erol.
Arthropod-Borne Diseases in the Lymelight
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- Emerging Fluorescence Technologies for Analysis of Protein Localization and Organelle Dynamics
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- - Lippincott-Schwartz, Jennifer.
National Institutes of Health (U.S.) (2010/03/04) - - Category : Wednesday Afternoon Lectures
- - Lippincott-Schwartz, Jennifer.
Emerging Fluorescence Technologies for Analysis of Protein Localization and Organelle Dynamics
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- A Family's Request for Complementary Medicine After Brain Death
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- - Applbaum, Arthur Isak.
National Institutes of Health (U.S.) (2010/03/04) - - Category : Clinical Center Grand Rounds
- - Applbaum, Arthur Isak.
A Family's Request for Complementary Medicine After Brain Death
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- Evolution and the Origins of Life
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- - Morowitz, Harold J.
National Institutes of Health (U.S.) (2010/03/04) - - Category : Evolution and Medicine
- - Morowitz, Harold J.
Evolution and the Origins of Life
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- NIH Diversity Seminar Series - April 2007 (NIH Only)
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- - Elias A. Zerhouni, M.D., Director, National Institutes of Health (2010/03/04)
- - Category : NIH Only
- The NIH Diversity Seminar Series will provide tools for incorporating concepts of diversity in the workplace and a resource for managing a multicultural workforce. In addition, the seminar will provide managers and supervisors with tangible tools and information to help fulfil their workforce diversity performance standards.
Keynote Speaker: Elias A. Zerhouni, M.D. Director, National Institutes of Health
NIH Diversity Seminar Series - April 2007 (NIH Only)
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- In Vivo Imaging of CD8 T Cell Interactions After Vaccinia Virus Infection
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- - Hickman-Miller, Heather.
National Institutes of Health (U.S.). Infectious Disease Imaging Interest Group. (2010/03/04) - - Category : Infectious Disease Imaging
- - Hickman-Miller, Heather.
In Vivo Imaging of CD8 T Cell Interactions After Vaccinia Virus Infection
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- Mechanistic Studies of RNAP II Transcription Elongation (NIH-Only)
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- - Jones, Katherine A.
National Institutes of Health (U.S.) (2010/03/04) - - Category : NCI CCR Grand Rounds (NIH Only)
- - Jones, Katherine A.
Mechanistic Studies of RNAP II Transcription Elongation (NIH-Only)
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- Emerging Role of Mass Spectrometry in Structure Elucidation of Cellular Complexes
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- - Michal Sharon, PhD Department of Chemistry, Cambridge University (2010/03/04)
- - Category : Proteomics
- High throughput proteomic approaches have identified numerous in vivo protein complexes and suggested the existence of many others from extensive protein interaction networks. Standard proteomics techniques are however unable to describe the stoichiometry, subunit interactions and organisation of these assemblies since many are heterogeneous, present at low cellular abundance and frequently difficult to isolate. We have developed mass spectrometry of non-covalent complexes for the study of novel protein assemblies isolated directly from the cellular environment. This approach is yielding not only detailed information about subunit stoichiometry, composition and dynamic interactions but also the overall architecture of multiprotein complexes.
For more information, visit
http://proteome.nih.gov
Emerging Role of Mass Spectrometry in Structure Elucidation of Cellular Complexes
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- Stopping the Clock on Diabetes in Women: Strategies for Prevention and Treatment Across the Lifespan
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- - Rodgers, Griffin P.
National Institutes of Health (U.S.) (2010/03/04) - - Category : Women's Health
- - Rodgers, Griffin P.
Stopping the Clock on Diabetes in Women: Strategies for Prevention and Treatment Across the Lifespan
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- NCI Board of Scientific Advisors - November 2006 (Day 1)
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- - NCI Board of Scientific Advisors. Meeting 2006 : (2010/03/04)
- - Category : Advisory Boards
NCI Board of Scientific Advisors - November 2006 (Day 1)
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- Redox Biology: Immunology - Epidemiology
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- - Espey, Michael Graham.
National Institutes of Health (U.S.) (2010/03/04) - - Category : Redox Biology
- - Espey, Michael Graham.
Redox Biology: Immunology - Epidemiology
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- Role of X Chromosomes in Women's Health and Sex-Specific Diseases
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- - Migeon, Barbara R.
National Institutes of Health (U.S.) (2010/03/04) - - Category : Women's Health
- - Migeon, Barbara R.
Role of X Chromosomes in Women's Health and Sex-Specific Diseases