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지식나눔

지식나눔

mixed lymphocyte reaction에 대해

2007-04-30 org.kosen.entty.User@7c9bf07c 윤진선(shin4537)
  • 1
면역학 실험 초자인데요.. 혹시 이 실험 방법 프로토콜 가지고 계신분 있으신가요? 아니면 실험할 때 주의사항이라든지요... 고수님들 좀 도와주세요^^
  • MLR
지식의 출발은 질문, 모든 지식의 완성은 답변! 
각 분야 한인연구자와 현업 전문가분들의 답변을 기다립니다.
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    홍동호님의 답변

    2007-07-20
    • 0

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    Assays of mixed lymphocyte response (MLR) and cytotoxicity Freshly harvested splenocytes from C57BL/6 and BALB/c mice were used as stimulator and responder cells, respectively. Stimulator cells were resuspended and treated with 50 g/ml mitomycin C (as a final concentration, Sigma) at 37oC for 30 min to inhibit their proliferation. Cells were quickly washed twice with RPMI 1640, combined with responder cells in 96-well plates at a 4:1 ratio (5 × 105 cells:1.25 × 105 cells; stimulator:responder) with various concentrations of CT compounds and cultured for 3 days. The proliferation of lymphocytes was measured by cell proliferation assay kit-8. For cytotoxicity assay, splenocytes from BALB/c mice were cultured in 96-well plates with various concentrations of CT compound in the presence of 5 g/ml pokeweed mitogen (Sigma) for 3 days. Cell proliferation was measured by cell proliferation assay kit-8. > >면역학 실험 초자인데요.. > >혹시 이 실험 방법 프로토콜 가지고 계신분 있으신가요? > >아니면 실험할 때 주의사항이라든지요... > >고수님들 좀 도와주세요^^
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    Assays of mixed lymphocyte response (MLR) and cytotoxicity Freshly harvested splenocytes from C57BL/6 and BALB/c mice were used as stimulator and responder cells, respectively. Stimulator cells were resuspended and treated with 50 g/ml mitomycin C (as a final concentration, Sigma) at 37oC for 30 min to inhibit their proliferation. Cells were quickly washed twice with RPMI 1640, combined with responder cells in 96-well plates at a 4:1 ratio (5 × 105 cells:1.25 × 105 cells; stimulator:responder) with various concentrations of CT compounds and cultured for 3 days. The proliferation of lymphocytes was measured by cell proliferation assay kit-8. For cytotoxicity assay, splenocytes from BALB/c mice were cultured in 96-well plates with various concentrations of CT compound in the presence of 5 g/ml pokeweed mitogen (Sigma) for 3 days. Cell proliferation was measured by cell proliferation assay kit-8. > >면역학 실험 초자인데요.. > >혹시 이 실험 방법 프로토콜 가지고 계신분 있으신가요? > >아니면 실험할 때 주의사항이라든지요... > >고수님들 좀 도와주세요^^
    등록된 댓글이 없습니다.
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